Indicators on high performance liquid chromatography method You Should Know
For this experiment, the mobile phase is primarily 20% acetonitrile and 80% purified deionized (DI) drinking water. A small number of acetic acid is added to decrease the pH of your cellular section, which keeps the silanol in the stationary packing section within an undissociated state.The elution purchase of solutes in HPLC is ruled by polarity.